Skip to main content
. 2020 Dec 9;11(12):1043. doi: 10.1038/s41419-020-03263-6

Fig. 5. Involvement of HDAC3 in kcnq1ot1-induced downregulation of ABCA1 expression.

Fig. 5

A, B THP-1 macrophages were transfected with PBS, LV-NC or LV-kcnq1ot1 for 72 h. The expressions of LXRα, HDAC1, HDAC3, HDAC5, and HAT1 were determined by qRT-PCR and western blot (n = 3). C, D Evaluation of HDAC3 expression in the aorta from apoE−/− mice (n = 10). E, F Detection of HDAC3 expression in MPMs from apoE−/− mice. G THP-1 macrophages were transfected with scrambled siRNA or HDAC3 siRNA for 24 h, and the cell lysates were immunoblotted with indicated antibodies (n = 3). H–J THP-1 macrophages were transfected with HDAC3 siRNA for 24 h, followed by treatment with LV-kcnq1ot1 for another 72 h (n = 3). H, I The mRNA and protein levels of ABCA1 were detected by qRT-PCR and western blot, respectively. J Detection of cholesterol efflux to apoA-I using a liquid scintillation counter. Data are represented as mean ± SD. **P < 0.01, ***P < 0.001 vs. control group; ##P < 0.01, ###P < 0.001 vs. LV-kcnq1ot1 group; ns not significant.