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. 2020 Dec 9;11(12):1043. doi: 10.1038/s41419-020-03263-6

Fig. 6. Identification of HDAC3 as a direct target of miR-452-3p.

Fig. 6

A Schematic of miR-452-3p binding site in the 3′UTR of HDAC3 mRNA and corresponding mutation. B The luciferase reporter plasmids (HDAC3-WT and HDAC3-Mut) were co-transfected into 293T cells with miR-452-3p mimic or its negative control. Luciferase activity was measured after 48 h. C–E THP-1 macrophages were transfected with mimic/inhibitor or their negative controls for 48 h; C qRT-PCR analysis of miR-452-3p expression. D, E The mRNA and protein levels of HDAC3 were determined by qRT-PCR and western blot, respectively. Data are represented as mean ± SD from three independent experiments. **P < 0.01, ***P < 0.001; ns not significant.