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. 2020 Oct 8;15(1):986–996. doi: 10.1515/med-2020-0219

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© 2020 Lili Zhou et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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Knockdown of FGF12-AS2 significantly inhibited the proliferation of NSCLC cells. A549 or NCI-H23 cells were transfected with FGF12-AS2 shRNA1 or FGF12-AS2 shRNA2 for 24 h. Then, the expression of FGF12-AS2 in (a) A549 and (b) NCI-H23 cells was detected by q-PCR. (c and d) A549 or NCI-H23 cells were transfected with nothing, NC or FGF12-AS2 shRNA2 for 0, 24, 48, or 72 h. Then, the cell viability was detected by the CCK-8 assay. (e) The proliferation of A549 cells was tested by Ki-67 staining. Red immunofluorescence indicated Ki-67. Blue immunofluorescence indicated DAPI. (f) The positive rate of Ki-67 staining was calculated. (g) The proliferation of NCI-H23 cells was measured by Ki-67 staining. Red immunofluorescence indicated Ki-67. Blue immunofluorescence indicated DAPI. (h) The positive rate of Ki-67 staining was calculated. ^** P < 0.01 compared to control.