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. 2020 Dec 2;40(49):9533–9546. doi: 10.1523/JNEUROSCI.0407-20.2020

Figure 3.

Figure 3.

CRTC2 plays an important protective role in endothelial function. A, Quantitative results of the relative permeability ratio (n = 6–10). B, Quantitative results of the relative permeability ratio (n = 9). C, The expression level of CRTC2 in BBMCs overexpression of CRTC2 compared with those transfected with EGFP (control) is shown. D, The expression level of CRTC2 in BBMCs transfected with LacZ-specific (miR-Cont) or CRTC2-specific (miR-CRTC2) miRNAi-expressing adenovirus is shown. E, Representative image of tube-like capillary formation in Matrigel by BBMCs transfected with EGFP or CRTC2. Scale bar: 100 µm. F, Quantitative results of the relative tube length (n = 6). G, Quantitative results of relative tube length (n = 6). H, Representative migration assay image of BBMCs. Scale bar: 100 µm. I, Quantitative results of the relative migration distance (n = 5). J, Quantitative results of relative migration distance (n = 6). K, Quantitative results of the relative proliferation ratio (n = 6). L, Quantitative results of the relative permeability ratio under ischemic conditions (n = 5 or 6). M, Quantitative results of the relative permeability ratio under ischemic conditions (n = 5 or 6). Error bars represent mean ± SEM. For A, B, L, M, *p < 0.05 by one-way ANOVA, followed by Tukey−Kramer post hoc analysis. For F, G, I, J, *p < 0.05 compared with EGFP by two-tailed unpaired Student's t test. For K, NS indicates no significant change by two-tailed unpaired Student's t test.