Figure 5.

Ser³⁰⁷ in CRTC2 is related to its nuclear localization. A, The bEnd.3 cells were cotransfected with the each CRTC2WT, CRTC2 S307G, CRTC2ΔNES, CRTC2 S171A adenovirus vector. CRTC2 protein was visualized by immunofluorescence. Scale bar: 50 µm. B, Four types of bEnd.3 cells were classified into three categories located in cytoplasma (C), in both cytoplasma and the nucleus (C&N), and in the nucleus (N). C, Diagrams of CRTC2 mutants used for assays of intracellular distribution. The HUVECs transformed with pEGFP-CRTC2 WT, CRTC2 S307G, CRTC2 S171A, or CRTC2 ΔNES adenovirus vector. Scale bar: 50 µm. D, Four types of HUVEC cells were classified into three categories located in cytoplasma (C), in both cytoplasma and the nucleus (C&N), and in the nucleus (N). E, The bEnd.3 cells were cotransfected with adenovirus expressing CRTC2 WT- or CRTC2 containing a triple mutation at Ser¹⁷¹, Ser²⁷⁵, and Ser³⁰⁷. The localization of CRTC2 was examined by immunofluorescence. Scale bar: 50 µm. F, Two types of bEnd.3 cells were classified into three categories located in cytoplasma (C), in both cytoplasma and the nucleus (C&N), and in the nucleus (N).