Figure 6.

Neonatal Jag1 deletion from pillar and Deiters' cells caused hearing deficits in the low-frequency range. A, Experimental strategy. Fgfr3-iCreER^T2::Jag1^fx/fx (Jag1 CKO) and Jag1^fx/fx (Ctrl) mice were injected with tamoxifen at P0/P1, followed by ABR at P30, and analysis of the cochlea at P30 or P60. B, C, Quantification of IHCs, OHCs, and individual SC subtypes (inner pillar cells to Hensen's cells) in Ctrl and Jag1 CKO mice at P30 (B) and P60 (C). Data are mean ± SEM; n = 4/group. ****p < 0.0001. D, ABR thresholds of Ctrl and Jag1 CKO at P30. Data are mean ± SEM; n = 8-10/group. **p < 0.01; two-way ANOVA followed by a Bonferroni post hoc test. DC1, Deiters' cell row 1; DC2, Deiters' cell row 2; DC3, Deiters' cell row 3; HeC, Hensen's cell; IPC, inner pillar cells; OPC, outer pillar cells.