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. 2020 Dec 2;40(49):9455–9466. doi: 10.1523/JNEUROSCI.2069-20.2020

Figure 5.

Figure 5.

Number of ARH GHRHCre/+-eGFP and Kiss1Cre-eGFP neurons increases during pubertal transition. A–D, Fluorescent micrographs showing distribution of GHRHCre/+-eGFP+ in the ARH at prepubertal (P21) and adult (P60) ages in males (A,B) and females (C,D). E, Quantification of number of ARH GHRH-eGFP+ neurons during pubertal transition in males and females (F(1,16) = 6.6, p = 0.021). F–I, Fluorescent micrographs showing distribution for ARH Kiss1Cre-eGFP+ cells at prepubertal (P23) and adult males (F,G); and prepubertal (P15) and diestrus females (H,I). J, Quantification of number of ARH Kiss1-eGFP+ neurons during pubertal transition in males and females (F(1,15) = 38.7, p < 0.0001; n = 4-6/group). Each point represents 1 individual mouse. *p < 0.05 versus prepubertal; #p < 0.05 versus adult males; two-way ANOVA with Tukey's post hoc analysis. 3v, Third ventricle. Scale bar, 50 µm.