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. 2020 Nov 20;23(12):101829. doi: 10.1016/j.isci.2020.101829

Figure 3.

Figure 3

Microglial Oxidative Stress, Function, and Reactivity Across Chronological and Endocrinological Aging

(A–F) Figures A–F are representative figures of the gating strategy utilized for the assessment. Microglia were selected from the single cell suspension by first gating for (A) total cells from the suspension, then (B) singlets, and (C) then gating for microglia using CD11b (FITC) high and CD45 (Vio-Blue) intermediate as a gating strategy. Microglial cells expressing CD11b (FITC) were used for gating for (D) mitochondrial oxidative stress (PE) (E) output of phagocytic capacity (PE) and (F) MHC-II expression (PE-Vio770).

(G–I) (G) Quantification of mitochondrial oxidative stress. (H) Quantification of phagocytic capacity. (I) Quantification of MHC-II expression. Data are represented as mean ± SEM. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001 calculated using one-way ANOVA.