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. 2020 Nov 23;23(12):101851. doi: 10.1016/j.isci.2020.101851

Figure 1.

Figure 1

TGF-β Induces Myofibroblastic-Like Phenotype of the Hepatic Stellate Cells (LX-2s) within the Organoid

(A) LX-2 organoids are grown for 7 days with or without 10 ng/mL TGF-β and IHC stained for α-SMA or FAP (green), DAPI (blue), and Ki-67 (red).

(B) LX-2 activation was determined by IHC quantification of FAP, a-SMA, and Ki-67 using VisioPharm software by calculating the percentage of positively expressed cells by the total nuclei present. Graphs represent mean ± s.e.m (n = 5) and generated using GraphPad prism.