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. 2020 Nov 23;23(12):101851. doi: 10.1016/j.isci.2020.101851

Figure 8.

Figure 8

Organoid with Dense ECM Reduces Tumor Cell Response to Specific Fluorouracil-Based Chemotherapy

(A) Organoids were treated with FOLFIRI (5-FU, 1mM; Leucovorin, 20 μM; Irinotecan, 50 μM), FOLFOX (5-FU, 1mM; Leucovorin, 20 μM; Oxaliplatin, 50 μM), 5-FU (1mM) or Control (DMSO) for 72hr after organoid was allowed to self-organize for 96hr. Paraffin sections were stained with DAPI (blue), Ki-67 (red), and Caspase-3 (green) where the HCT-116 cells can be seen within the spheroid (white dashed circle) and the LX-2s are surrounding.

(B–E) Immunofluorescent images of treated organoids were analyzed using VisioPharm to determine total HCT-116 (dash gray line) caspase-3 to Ki-67 ratio in each drug condition. TGF-β inhibitor was giving at 24hr post TGF-β addition. Graphs represent mean ± s.e.m (n = 12) and generated using GraphPad prism. (∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001; ∗∗∗∗p ≤ 0.0001).