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Level of IL-8 expression in IL-8 manipulated cells. (A, B) RT-qPCR analysis of IL-8 mRNA expression following transfection with IL-8 knockdown in MDA-MB-231 cells and IL-8 overexpression in MCF-7 cells compared with control cells. (C, D) Results from ELISA of IL-8 protein secretion in IL-8 knockdown and IL-8 overexpression groups. (*** P < 0.001).
