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. 2020 Dec 7;19:1533033820979672. doi: 10.1177/1533033820979672

Figure 4.

Figure 4.

Association between IL-8 and miR-200 family, and effect of miR-200 family on IL-8-induced EMT. (A) RT-qPCR was performed to detect miR-200 family expression in MCF-7 cells compared with MDA-MB-231 cells. (B, C) MiR-200 family expression in IL-8 knockdown and IL-8 overexpression groups compared with respective controls by RT-qPCR analysis. (D) MCF-7 cells were transfected to overexpress IL-8, then miR-200b and miR-200c mimics were transfected compared with miR-NC. (E) MCF-7 cells were treated with human IL-8 at a concentration of 15 ng/ml for 48 h. Then miR-200b mimic was transfected compared with miR-NC. After 72 h, western blot was performed to demonstrate the expression level of EMT biomarkers. (***P < 0.001).