Figure 6.

Effect of GB on the stemness of SKOV3 via the PAF/PAFR pathway in vivo. A. Representative photographs of tumors extracted from mice treated with or without GB (1 mg/mice, i.p., every day for two weeks). B. The ovarian tumor volume was determined in mice administered GB. C. The weight of each mouse was recorded 3 times per week. The mice in the GB group had higher weights than the mice in the DMSO group. D. GB decreased the CD44+CD133+ cells percentage, and no obvious side effects were observed in the mice treated with GB. E. Protein expression of Ki67 and CD34 in ovarian cancer tissues by immunohistochemistry, the expression of Ki67 and CD34 was decreased in the GB-treated group, which indicated GB inhibited cell proliferation and angiogenesis in the ovarian cancer cells in vivo. All values are expressed as mean ± SD and compared by Student’s t test. All experiments were repeated three times. *, P < 0.05; **, P < 0.01.