MALAT-1 enhanced epithelial-mesenchymal transition (EMT) of EOC cells via miR22/c-myc axis. A: MALAT-1 shRNA, miR-22 mimics, or MALAT-1 and miR-22 mimics were introduced into SKOV3 cells. The c-myc RNA levels were examined by qPCR; B: The c-myc protein was detected using immunoblots and then quantified by gray-scale analysis; C: The epithelial-mesenchymal transition markers, including E-cadherin, vimentin, snai1, were detected by immunoblots and then quantified by gray-scale analysis. Data are expressed as mean ± standard deviation (x̅ ± sd) and represent three replicated experiments. *P<0.05 vs. the negative control (NC), and #P<0.05 vs. the miR-22 group. MALAT-1: Metastasis-associated lung adenocarcinoma transcript-1.