Figure 1.
Resection of primary B16 melanoma establishes immunological memory against re-challenged B16 melanoma. 5 × 105 B16F10 cells were subcutaneously injected in the right flank of B6 mice on day -31. The primary tumors were surgically removed on day -21. On day 0, the memory group of mice with the resection of primary tumors were re-challenged with 5 × 105 B16F10 cells in the left flank. Mice in the control group were also subcutaneously injected in the left flank with 5 × 105 B16F10 cells on day 0, but without primary tumor inoculation and resection. The growth of re-challenged tumors was monitored daily. A. Schematic of the experimental design. B. Tumor volumes of the re-challenged B16 melanoma. C. Representative images of mice with tumor outgrowth on day 16 post B16 cell re-challenge. Data are presentative of two independent experiments (mean ± SD, n=5). ***P < 0.001; unpaired student’s t-test.