Figure 3.

Pmel-1 CD8⁺ displays an effector T cell phenotype during killing of B16F10 cells. The freshly isolated or the activated Pmel-1 splenocytes were co-cultured with B16F10 target cells at a CD8⁺ effector to target ration of 40:1 for 10 hours, followed by flow cytometry analysis. A. Representative plots of CD44 and CD62L expressions on CD8⁺ T cells. B. Quantitation of % CD44⁺ cells among Pmel-1 CD8⁺ cells. C. Histograms show CXCR6, KLRG1, PD-1 and Ki67 expressions on Pmel-1 CD8⁺ T cells. D. Bar graphs display mean fluorescence intensity (MFI) of CXCR6, KLRG1, PD-1 and Ki67 of Pmel-1 CD8⁺ T cells; E. Representative contour plots show % IFN-γ⁺ and % perforin⁺GzmB⁺ cells among Pmel-1 CD8⁺ T cells; F. Quantitation of % IFN-γ⁺ and % perforin⁺GzmB⁺ cells among CD8⁺ T cells. Data are presented by mean ± SD (n=5). ***P < 0.001; unpaired student’s t-test.