Figure 4.

Adoptive transfer of Pmel-1 CD8⁺ Teff cells inhibits melanoma metastasis. WT B6 mice were intravenously injected with 0.25 × 10⁶ B16F10-Fluc cells, and adoptively transferred with (Teff group) or without (control group) 5 × 10⁶ in vitro generated Pmel-1 CD8⁺ Teff cells on the same day. Melanoma metastasis and animal survival was monitored. A. Schematic of the experimental design. B. Animal survival after B16F10-Fluc cell injection. C. Images show metastasis nodules on lungs on day 24 post B16F10-Fluc cell inoculation. D. Luminescence images of mice on days 14, 21, 35 and 49 post B16F10-Fluc cell inoculation. Data are presentative of two independent experiments (mean ± SD, n=5). **P < 0.01; log-rank test.