Figure 5.

Early adoptive Pmel-1 CD8⁺ Teff cell transfer inhibits later melanoma metastasis (model 1). WT B6 mice were adoptively transferred with 5 × 10⁶ in vitro generated Pmel-1 CD8⁺ Teff cells, and intravenously injected with 0.25 × 10⁶ B16F10-Fluc cells either on the same day (Teff group) or 21 days later (Tmem group). Control WT B6 mice did not receive T-cell transfer, and were injected with B16F10-Fluc cells on the same day as either the Teff group (Control-A group) or the Tmem group (Control-B group). Post B16F10-Fluc cell injection, animal survival and tumor metastasis were assessed. A. Schematic of the experimental design. B. Animal survival post B16F10-Fluc cell injection. C. Luminescence images of mice on days 14, 21, 35 and 49 post B16F10-Fluc cell inoculation. Data are presentative of two independent experiments (mean ± SD, n=5). **P < 0.01; log-rank test.