Figure 6.

Early adoptive Pmel-1 CD8⁺ Teff cell transfer inhibits later melanoma metastasis (model 2). WT B6 mice were intravenously injected with 0.25 × 10⁶ B16F10-Fluc cells on day 0. The Teff group or Tmem group of mice were adoptively transferred with 5 × 10⁶ Pmel-1 CD8⁺ Teff cells either on day 0 or day -21, receptively. The control group did not receive T-cell transfer and were only injected with B16F10-Fluc cells. A. Schematic of the experimental design. B. Animal survival post B16F10-Fluc cell injection. C. Luminescence images of mice on days 21, 28, 35 and 49 post B16F10-Fluc cell inoculation. Data are presentative of two independent experiments (mean ± SD, n=5). **P < 0.01; log-rank test.