Table 3.

Combination Index (CI) for HEP3B (liver cancer cells) treated with combination of different agents

Agent 1	Conc. µg/ml	Agent 2	Conc. mg/ml	CI
BR	15	NAC	2.45	0.36
	25			0.39
Agent 1	Conc. µg/ml	Agent 2	Conc. mg/ml	CI	Agent 3	Conc. µg/ml	CI	Combo (3 agents)	CI
GEM	0.026	NAC	2.45	-	BR	7.0	0.86	GEM + NAC + BR	0.23
	0.131			-			-		0.21
	0.263			-			0.88		0.18
	0.065			-			-		0.16
	1.315			0.59			0.45		0.19
	2.63			0.32			0.52		0.23
	6.58			0.6			0.85		0.32
5-FU	0.0650	NAC	2.45	-	BR	7	0.8	5-FU + NAC + BR	0.43
	0.130			-			0.68		0.18
	0.325			-			0.68		0.22
	0.650			0.59			0.72		0.17
	1.3			0.32			0.88		0.26
	2.0			0.6			-		0.17
	2.6			-			-		0.24
OXALI	3.97	NAC	2.45	-	BR	7.0	0.99	OXALI + NAC + BR	-
	5.96			-			0.54		-
	9.93			-			0.69		-
CIS	1.5			1.0			-		-
	3.0			0.61			0.52		0.66
	6.0			-			0.77		-
Agent 1	Conc. ng/ml	Agent 2	Conc. mg/ml	CI	Agent 3	Conc. µg/ml	CI	Combo (3 agents)	CI
DOX	0.54	NAC	2.45	-	BR	10	0.68	DOX + NAC + BR	0.85
	5.43			-			0.79		0.81
	27.18			0.83			0.63		0.83
	54.35			0.66			0.6		-
	272			0.55			0.65		0.97
	544			0.34			0.48		0.69
	1359			0.74			0.88		-

CI values with 1.0 or >1.0 is not shown in the table. CI values <0.5 are highlighted to denote good synergy.