Figure 1.

PD Stressors treatment in differentiated N27-A cells increases cell death. A. Top: Flow cytometry with unstained (blank, blue cells), Annexin V only (early apoptosis, green cells), and 7-aminoactinomycin D (7-AAD) only (dead cells and/or late apoptosis, red cells) used for calibration. Bottom: flow cytometry analysis with Annexin V/7-AAD was used to assess cell death after dopamine (250 μM) treatment, rotenone (1 μM), versus vehicle (0.01% DMSO). Values are mean ± SEM; *P < 0.001, n = 9/group. B. Bar graph showing an increase in early apoptosis (Annexin V+) in DA and Rot treated cells compared with Vehicle. Values are expressed as means ± SEM, *P < 0.001, n = 9/group. C. Bar graph showing an increase in cell death (7-AAD+) in DA and Rot treated cells compared with Vehicle (Veh). Values are expressed as means ± SEM; *P < 0.001, n = 9/group. D. Bar graph showing a reduction in live in DA and Rot treated cells compared to Vehicle Values are expressed as means ± SEM, *P < 0.001, n = 9/group. E. Bar graph showing a reduction in cell viability (MTT assay) in DA and Rot treated cells compared with sham. Values are expressed as means ± SEM of five independent experiments, *P < 0.001, n = 9/group.