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. 2020 Nov 15;12(11):7542–7564.

Figure 8.

Figure 8

Parkin knockdown attenuates Rot-, but not DA-induced neuron death. A. Top: Immunoblot showing the reduction in expression of Parkin in N27-A cells after transfection with Parkin siRNA versus scrambled siRNA. Bottom: Graph showing a decrease in Parkin expression. Values are expressed as means ± SEM of three independent experiments, *P < 0.05 Parkin siRNA-treated cells versus cells treated with scrambled siRNA (n = 9/group). B. Graph showing the level of cell death after transfection with scrambled siRNA or Parkin siRNA, and treatment with DA, or Rot. Values are expressed as means ± SEM of five independent experiments, *P < 0.001 Parkin siRNA-treated cells versus cells treated with scrambled siRNA (n = 9/group). C. Bar graph showing the level of cell death after overexpression of Myc-USP30, and treatment with DA, or Rot. Values are expressed as means ± SEM of five independent experiments, *P < 0.001. Note that the cell death in Rot treatment resulted in less cell death in USP30 expressing cells.