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. 2020 Nov 15;12(11):7542–7564.

Figure 9.

Figure 9

DA, but not Rot, treatment induces activation of AIF-PARP pathway, and increase in calpain activity. A. Western blot analysis and graph showing an increase in apoptosis-inducing factor (AIF) expression in N27-A cells treated with DA compared with Vehicle-, and Rot-treated cells. Values are expressed as means ± SEM of five independent experiments, *P < 0.001 (n = 5/group). B. Immunoblots and graph showing an increase in poly(ADP-ribose),polymerase (PARP) expression in N27-A cells treated with DA compared with Vehicle-, and Rot-treated cells. Values are expressed as means ± SEM of three independent experiments, *P < 0.001 (n = 5/group). C. Graph showing the increase in calpain activity in N27-A cells treated with DA compared with Vehicle-, and Rot-treated cells. Note: Calpain activity was measured using a kit in the presence or absence of calpain substrate and inhibitor. Values are expressed as means ± SEM of five independent experiments, *P < 0.001 (n = 5/group).