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In vitro model of ALS was successfully established. NSC-34 cells were treated with hTBK1-c.978T>A mutation or NC for 0, 24, 48 or 72 h. A. Cell viability was tested by CCK-8 assay. B. The proliferation of NSC-34 cells was measured by Ki67 staining. Red indicates Ki67; Blue indicates DAPI. C. The positive cell rate of Ki67 staining was calculated. **P < 0.01 compared to control, n = 3.
