Table 1.
Details of the primary and secondary antibodies in this study.
| Primary antibody | Immunogen | Manufacturing details | Specificity/characterization | Dilution |
|---|---|---|---|---|
| Acid sensing ion-channel 3 (ASIC3) | Corresponding to residues 285-304 of the extracellular domain rat ASIC3 | Neuromics; guinea pig polyclonal; #GP14105 | 41-43 | 1:300 |
| Calcitonin gene-related peptide (CGRP) | Synthetic rat CGRP conjugated to KLH | Sigma-Aldrich; rabbit polyclonal; #C8198 | 34,35 | 1:1000 |
| Neurofilament 200 (NF200) | Carboxyterminal tail segment of pigneurofilament H-subunit | Sigma-Aldrich; mouse monoclonal; #N0142 | 36 | 1:1000 |
| Tyrosine receptor kinase A (TrkA) | Purified recombinant rat TrkA extracellular domain (Ala33-Pro418) | R&D Systems; goat polyclonal; #AF1056 | 37 | 1:1000 |
|
Transient receptor potential Vanilliod 1 (TRPV1) |
Intracellular C-terminus of rat TRPV1 (824-838) |
Alomone Labs; rabbit polyclonal; #ACC-030 |
38,39 |
1:1000 |
|
Secondary antibody |
Manufacturing details |
Dilution |
||
| Donkey anti-Goat Alexa Flour 594 | Jackson Immuno- Research, #705-585-147 | 1:200 | ||
| Donkey anti-Guinea Pig Alexa Flour 488 | Jackson Immuno- Research, #706-545-148 | 1:200 | ||
| Donkey anti-Mouse Alexa Flour 647 | Molecular probes, Invitrogen, #A31571 | 1:200 | ||