Figure 1.

Increased EHMT Activity during Early Stages of OKSM-Driven Reprogramming

(A) Representative bright-field and fluorescence images of MEFs and cells under the indicated reprogramming conditions 24 h after induction of OKSM and staining with antibodies against EHMT1 (top) or EHMT2 (bottom).

(B) Mean fluorescence intensities after IF with antibodies against EHMT1 and EHMT2 under the indicated conditions. At least 100 size-matched nuclei were analyzed. Similar results were obtained in three independent experiments.

(C) Representative mean fold change in EHMT1 and EHMT2 protein levels analyzed by WB during basal and enhanced reprogramming. n = 3 independent experiments.

(D) Same as (B) but after IF against indicated histone marks.

Significance in (B–D) with one-way ANOVA with Tukey post test: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001.