Figure 2.

EHMT Activity Supports 3c Enhanced Reprogramming

(A) Alkaline phosphatase staining of iPSCs formed from the indicated input MEFs via basal and 3c enhanced reprogramming in the absence and presence of UNC0638, respectively. Images were taken 4 days after dox was removed to select for stably reprogrammed cells.

(B) Quantification of iPSC colony formation under the indicated conditions as percentage input MEFs. n = 3 independent experiments. Significance with unpaired t test: ^∗∗∗p < 0.001.

(C) Percentage iPSC colonies formed from reprogrammable MEFs under basal or 3c enhanced conditions that were exposed to EHMT inhibitor during the indicated time windows (“+” indicates chronic exposure and “−” addition of DMSO instead of UNC0638). Input cells were 2,500 (basal) and 300 (3c enhanced) MEFs, respectively.

n = 3 independent experiments. Significance with one-way ANOVA with Dunnett post test: ^∗∗p < 0.01 and ^∗∗∗p < 0.001.