Figure 4.

Impact of EHMT Inhibition on Later Stages of iPSC Reprogramming

(A) Representative images of colonies formed upon 4 days of OKSM expression under 3c conditions in the absence (DMSO) or presence of UNC0638. Starting MEFs were labeled with a lentiviral vector expressing EGFP to visualize colony morphology.

(B) Quantification of day 4 colonies with mesenchymal or epithelial morphology under the indicated conditions. Significance with multiple two-tailed t tests: ^∗∗p < 0.01 and ^∗∗∗p < 0.001. At least 100 colonies were scored in three separate reprogramming experiments for each condition.

(C) Relative abundance in the presence versus absence of UNC0638 (UNC) of the indicated reprogramming intermediates during 3c enhanced reprogramming, as measured by flow cytometry. n = 3 independent experiments.

(D) Same as (C) for reprogramming intermediates during basal reprogramming. n = 3 independent experiments. Significance in (C and D) with multiple t tests using Holm-Sidak correction: ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001.

(E) Percentage of stable iPSC colonies (per input MEFs) formed under 3c reprogramming conditions from cells transduced with indicated shRNAs.

(F) Like (E) but under basal reprogramming conditions.

Significance in (E and F) was calculated with one-way ANOVA with Tukey post test: ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗∗p < 0.0001. n = 3 independent experiments.