Figure 3.

MeCP2 Spatially and Physically Interacts with the MajSat Transcripts

(A) Representative images of undifferentiated TK23_WT cells and mESC-derived TK23_WT neurons examined by immuno-RNA FISH using MajSat-fw LNA probe and anti-MeCP2 antibody. Chromocenters were stained with DAPI. Merge: MeCP2/MajSat-fw RNA signals and MeCP2/MajSat-fw RNA/DAPI signals. Insets show magnifications of a chromocenter. Scale bar, 15 μm.

(B and C) RNA immunoprecipitation of MajSat transcripts in mESC-derived Mecp2^−/y (negative control) and TK23_WT neurons (B), and in total brain from Mecp2^−/y (negative control) and WT mice (C), using an anti-MeCP2 antibody.

Data are means ± SD of four independent qPCR replicates from two RIP experiments. ^∗∗∗p < 0.001; ^∗∗p < 0.01; NS, not significant (one-tailed Student’s t test). See also Figure S2.