Table 2. De novo assembly statistics for Pst inoculated/infected wheat transcriptome.
| Samples | Number | Length (nt) | Mean length (nt) | N50 | Distinct clusters | Distinct singleton | |
|---|---|---|---|---|---|---|---|
| Contig | Avocet-Yr10_Pst | 105866 | 23189064 | 219 | 249 | - | - |
| Avocet-S_Pst | 128680 | 29848685 | 232 | 272 | - | - | |
| Unigene | Avocet-Yr10_Pst | 55231 | 18753197 | 340 | 371 | 12956 | 42275 |
| Avocet-S_Pst | 68568 | 24329132 | 355 | 394 | 13564 | 55004 | |
| All | 61105 | 26978874 | 442 | 490 | 15016 | 46089 |
Distinct clusters are similar (more than 70%) unigenes, and these unigenes may originate from the same gene or homologous gene, whereas distinct singletons represent the unigenes come from a single gene.