Fig. 2.

NHLRC2 is significantly decreased in Nhlrc2^FINCA/− mouse tissues. SDS-PAGE immunoblotting showing NHLRC2 in hippocampus, cerebellum, and brainstem of 13-week-old and lung and liver of 32-week-old Nhlrc2^FINCA/− and Nhlrc2^+/+ mouse tissue lysates. The amount of NHLRC2 decreased to 1.1% in the hippocampus (p < 0.0001), 3.3% in the cerebellum (p < 0.0001), 6.2% (p = 0.0004) in the brainstem, 3.2% (p < 0.0001) in the liver, and 1.7% (p = 0.011) in the lung of Nhlrc2 ^FINCA/− mice compared to wild-type mice (Student’s t-test). The expected size of full length NHLRC2 is 78.4 kDa. Protein amounts are relative to one of the wild-type samples, and GAPDH was used for normalization. Dot blots show individual data points, group mean and standard error of the mean (SEM). ****p < 0.0001, ***p < 0.001