Fig. 2.

Detergent extraction of placental CSPGs. Intensity traces of the CS-specific oxonium ion m/z 362.11 in human placental samples after extraction either with sodium dodecyl sulfate (SDS) (A) or with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and fractionation on a column with immobilized rVAR2 (B). The major intensity differences were observed for CS glycopeptides eluting between 30 and 50 min (highlighted with a dashed box) corresponding to glycosites derived from the membrane-anchored proteoglycans CD44, CSPG4, GPC3 and SDC1 (marked in red).