Fig. 6.

Kinetic comparison of fucosylation and sialylation on Lewis X (Le^x) and sialyl Lewis X (sLe^x) synthesis. (A) Schematic view of the enzymatic steps for Le^x and sLe^x synthesis on the antibody glycan G0. The glycan short names follow that of Figure 3. (B) Kinetic comparison of galactosylation on G0 by B4GalT1, sialyation on G2 by ST3Gal4 and ST6Gal1 and fucosylation on A2[3] by FUT7. The reaction time for B4GalT1 was 10 min. The reaction time for ST3Gal4, FUT7 and ST6Gal1 was 5 h. In each case, the labeling enzyme was subject to a 3-fold serial dilution starting from 1 μg each of B4GalT1, FUT7 and ST6Gal1 and 3 μg ST3Gal4. (C) Kinetic comparison of sLe^x synthesis by FUT6 and FUT7 from A2[3]. Reaction time was 20 min. Both FUT6 and FUT7 were subject to 6-fold serial dilution starting from 1 μg. (D) Kinetic comparison of Le^x synthesis by FUT6 and FUT9 from G2. Reaction time was 20 min. Both FUT6 and FUT9 were subject to 6-fold serial dilution starting from 1 μg.