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. 2020 May 2;30(12):970–980. doi: 10.1093/glycob/cwaa030

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© The Author(s) 2020. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 7 — Quantification of sialyl Lewis a (sLe^a) and its intermediate products. (A) scheme of the synthesis of sLe^a using B3GalT2, ST3Gal3 and FUT3. (B) The step-by-step synthesis of sLe^a and a 2-fold serial dilution of G0f. Lane 1 is labeled G0 (G0f) and lane 2 is labeled G2 (G2f). Lanes 3–5 contain the intermediate reactions of converting G0f to the final sLe^a carrying glycan A1G1[3]F1f with the indicated enzymes. Lanes 6–13 is a 2-fold serial dilution of G0f starting from 25 ng (19 pmol). The exposure time is 240 ms. The mass inputs of the serial dilution and all the band intensities were listed below the picture. (C) The response curve of the serial dilution in (B). The slope of the line represents the signal to mass ratio of G0f, which allows the quantification of the bands from lanes 1 to 5.