Figure 4.
Cohousing ovariectomized mice with sham-operated (SHAM) mice did not protect against the development of the ovariectomized (OVX) metabolic phenotype. A: Design of cohousing study with adult female mice housed 2 per cage in 3 different housing arrangements that resulted in 4 groups of mice (n = 10 per group): SHAM, OVX, SHAMch, and OVXch. B–F: Compared with SHAM mice, OVX mice had increased weight, luteinizing hormone (LH) levels, abdominal adiposity, fasting blood glucose (FBG), and fasting blood insulin (FBI) after 5 weeks. Compared with OVX mice, OVXch mice had similar weight, LH levels, abdominal adiposity, FBG, and FBI. Graph error bars represent standard error of the mean. Different letters indicate significant differences in a 1-way analysis of variance (ANOVA) or repeated-measures 2-way ANOVA followed by post hoc comparisons with the Tukey-Kramer honestly significant difference test; P < 0.05.