Fig. 3.

Putative plasmid transfer among K. pneumoniae isolates and between different bacterial genera. Solid arrows indicate putative intra-species transfer, whereas dashed arrows indicate inter-bacterial transfer. The putative inter-patient transfer is illustrated through a question mark. Note that from PT12 (PBIO1961), no additional carbapenem-resistant isolates were obtained and that no other ST307-positive patient was simultaneously present on the same ward whereas inter-patient transfer for PT20 (PBIO2014) is further supported by the epidemiologic data. Line colors match the colors of transferred virulence/resistance plasmids and are also shown next to the isolate names as circles. Closed circles—complete plasmid backbone (Megablast hits with identity ≥ 99%: coverage ≥ 99%). Open circles—incomplete plasmid backbone (Megablast hits with identity ≥ 99%: coverage > 77% and < 99% (Additional file 2: Table S4)). a The phylogenetic tree is based on distance-corrected MinHash dissimilarities between sequenced genomes and was inferred with FastME as part of JolyTree. Shown at the branches is the rate of elementary quartets (REQ) for values ≥ 0.75 (circle diameter). b Timeline figure of plasmid transfer between July and December 2019