Fig. 7.

AKT inhibitor reduces cell proliferation, increases cell apoptosis, induces cell cycle arrest at G0/G1 phase, and suppresses the expression of HDAC3, CDK2. HL60/ADR cells were incubated with an AKT inhibitor (MK2206-2HCL). (a) CCK-8 assays were used to assess cell proliferation ability. (b) Flow cytometry was used to analyze cell apoptosis. c Representative of flow cytometry plot for detection of apoptosis. d-e Cell cycles of HL60/ADR cells were analyzed by flow cytometry after PI staining. f is a representative of flow cytometry for cell cycle detection. g Expression levels of HDAC3, AKT, P21, and CDK2 were measured by RT-PCR. h Expression levels of HDAC3, P-AKT, AKT, P21, and CDK2 were measured by Western blot. GAPDH was used as an internal control. Data represents three independent experiments, results are shown in the format, mean ± S.D. (*P < 0.05, **P < 0.01, ***P < 0.001, NS: P > 0.05)