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. 2020 Dec 9;12:109. doi: 10.1186/s13098-020-00618-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — CDKN2B-AS1 was verified as a sponge for miR-15b-5p in HMCs. a Complementary binding sites within CDKN2B-AS1 and miR-15b-5p were predicted using the starBase database. b Dual-luciferase reporter assay was executed in HMCs cotransfected with miR-15b-5p or miR-NC and luciferase vectors containing WT-CDKN2B-AS1 or MUT-CDKN2B-AS1. c QRT-PCR was employed to assess the levels of miR-15b-5p in serum of 34 DN patients and 34 healthy controls, and U6 was selected as an internal reference. d Pearson’s correlation analysis displayed the correlation between CDKN2B-AS1 and miR-15b-5p in serum of DN patients. e QRT-PCR exhibited the expression of miR-15b-5p in HMCs after HG treatment, and U6 was selected as an internal reference. f QRT-PCR presented the expression of CDKN2B-AS1 in HMCs transfected with pcDNA or CDKN2B-AS1 under HG treatment, and GADPH was selected as an internal control. g QRT-PCR revealed the influence of CDKN2B-AS1 knockdown or CDKN2B-AS1 overexpression on the expression of miR-15b-5p in HMCs under HG treatment, and U6 was selected as an internal reference. The experiments were repeated 3 times, and data were presented as mean ± standard deviation. ***P < 0.001 and ****P < 0.0001