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. 2020 Dec 9;12:109. doi: 10.1186/s13098-020-00618-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — WNT2B was verified as a target for miR-15b-5p in HMCs. a Complementary binding sites between WNT2B and miR-15b-5p were predicted using the starBase database. b Dual-luciferase reporter assay revealed the luciferase activity in HMCs cotransfected with miR-15b-5p or miR-NC and luciferase vectors containing WNT2B 3′UTR-WT or WNT2B 3′UTR-MUT. c QRT-PCR exhibited the expression of WNT2B mRNA in serum of 34 DN patients and 34 healthy controls, and GADPH was selected as an internal control. d Pearson’s correlation analysis displayed the correlation between WNT2B mRNA and miR-15b-5p in serum of DN patients. e Western blotting exhibited the level of WNT2B protein in HG-induced HMCs, and GADPH was selected as an internal control. The experiments were repeated 3 times, and data were presented as mean ± standard deviation. ***P < 0.001 and ****P < 0.0001