Table 1.
Genomic categories that are mapped by sRNA reads by size-fractionated total RNA libraries (TAP cloning method)
| Size-fractionated total RNA libraries, 5′ P-independent cloning, WT | |||
|---|---|---|---|
| Categories | 15-30nt (27nt sRNAs) | 30-45nt # (27nt and 31nt sRNAs) | 3'-end trimmed * (31nt sRNAs trimmed off oligo-As) |
| Total reads | 624,954 | 411,419 | |
| Unique (unique/total reads) | 242,277 (38.8%) | 171,309 (41.6%) | 86,031 |
| tRNAa,S | 2,343 (1.0%) | 2,848 (1.7%) | 1,035 (1.2%) |
| rRNAa,S | 12,957 (5.3%) | 18,235 (10.6%) | 450 (0.5%) |
| LINEsa,AS | 16,424 (6.8%) | 8,492 (5.0%) | 23,999 (27.9%) |
| Map to rest of genomea | 172,261 (71.1%) | 55,703 (32.5%) | 36,651 (42.6%) |
| Map to predicted ORFsa,AS | 124,770 (51.5%) | 37,145 (21.7%) | 27,508 (32%) |
| Not mapped to genome (%) | 38,292 (15.8%) | 86,031 (50.2%) | 23,896 (27.8%) |
a Number of unique reads divided by total unique reads
S Most reads are in sense orientation
AS Most reads are in antisense orientation
# Only 27nt sRNAs can be mapped to the genome, not 31nt sRNAs
* The 31nt sRNAs (non-mapped reads in 30-45nt library) were trimmed from 3' end into 27nt size, then they can be mapped to the genome.