Table 2.
Genomic categories that are mapped by sRNA reads by EhAgo IP libraries
| 5′ P-independent cloning, α-Myc IP libraries | ||||
|---|---|---|---|---|
| Categories | Myc-EhAgo2-1 | Myc-EhAgo2-2 | Myc-EhAgo2-3 | Myc-EhAgo2-2△NLS-DR |
| Total reads | 1,944,425 | 2,298,495 | 1,705,678 | 1,498,720 |
| Unique (unique/total reads) | 419,279 (21.6%) | 599,121 (26.1%) | 241,012 (14.1%) | 391,003 (26.1%) |
| tRNAa,S | 4,407 (1.0%) | 4,944 (0.5%) | 1,919 (0.4%) | 2,028 (0.5%) |
| rRNAa,S | 80,844 (19.3%) | 79,488 (13.3%) | 47,678 (19.8%) | 14,344 (3.7%) |
| EhLINEsa,AS | 41,713 (9.9%) | 15,275 (2.5%) | 16,306 (6.8%) | 41,820 (10.7%) |
| Map to rest of genomea | 230,334 (54.9%) | 388,040 (64.8%) | 139,897 (58.0%) | 282,829 (72.3%) |
| Map to predicted ORFsa,AS | 168,968 (40.3%) | 284,402 (47.5%) | 109,020 (45.2%) | 213,451 (54.6%) |
| Not mapped to genome (%) | 61,981 (14.8%) | 111,374 (18.6%) | 35,212 (14.6%) | 49,982 (12.8%) |
a Number of unique reads divided by total unique reads
S Most reads are in sense orientation
AS Most reads are in antisense orientation