Table 3.
Antisense sRNAs mapped genes overlap among three EhAgo IP libraries (TAP and RppH) and size-fractionated total RNA libraries (TAP)
| Three categories of genes | ||||||
|---|---|---|---|---|---|---|
| Sense (AS/S < 0.5) | Mixed (AS/S 0.5-2) | Antisense (AS/S >2) | Antisense (overlap among three EhAgos) | |||
| Libraries | ||||||
| α-Myc IP library | RppH | Myc-EhAgo2-1 | 116 | 13 | 319 | 226 |
| Myc-EhAgo2-2 | 178 | 60 | 297 | |||
| Myc-EhAgo2-3 | 81 | 36 | 255 | |||
| Myc-EhAgo2-2△NLS-DR | 73 | 24 | 300 | |||
| TAP | Myc-EhAgo2-1 | 79 | 31 | 324 | 238 | |
| Myc-EhAgo2-2 | 48 | 25 | 298 | |||
| Myc-EhAgo2-3 | 28 | 19 | 256 | |||
| Total RNA | TAP | 15-30nt size fraction | 57 | 68 | 244 | |
| 30-45nt size fraction* | 72 | 52 | 242 | |||
| 30-45nt size fraction with 3' end trimming# | 57 | 24 | 234 | |||
* most mapped reads are in 27nt peak
# most mapped reads are in 31nt peak