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. 2020 Dec 9;11:527. doi: 10.1186/s13287-020-02048-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Cell adhesion markers and migration of UCB CD34+ cells expanded with hWJSCs and hWJSC-CM. A Note the percentage of the most primitive CD34+CXCR4+ cells in the hWJSCs and hWJSC-CM-expanded groups was closest to the initial seeding cell numbers on day 0 (dotted line) compared to the controls. B Note significantly greater percentage of N-cadherin+ cells after expansion of CD34+ cells with hWJSCs as compared to controls. C (a–c) Phase-contrast images of the bottom chamber of transwell migration assays. Note increased number of CD34+ cells that have migrated from the top to the bottom chamber when cultured in the presence of hWJSCs and hWJSC-CM compared to controls. (d–f) There was an increased number of N-cadherin positive cells among the migrated cells expanded with hWJSCs and hWJSC-CM compared to controls. D Note significantly greater viable CD34+ cell numbers (MTS assay) lying in the bottom chamber of transwell migration chambers after expansion with hWJSCs as compared to controls. All values are expressed as mean ± SEM of 3 biological samples with 3 replicates for each sample. *p < 0.05