Skip to main content
. 2020 Dec 9;20:1211. doi: 10.1186/s12885-020-07675-7

Table 1.

qRT-PCR primers for validating microarray gene expression

Primer orientation Primer sequence (5′-3′) Melting Temperature
(Tm0C)
GC%
RUNX3 Forward primer GACAGCCCCAACTTCCTCT 56.9 57.8
Reverse primer CACAGTCACCACCGTACCAT 57.0 55
TFF3 Forward primer TCAAGCCCCTGCAGGAAGCAG 62.4 61.9
Reverse primer GCCGGGAGCAAAGGGACAGA 62.4 65
β-actin Forward primer AGCACTGTGTTGGCGTACAG 57.9 55
Reverse primer CTCTTCCAGCCTTCCTTCCT 56.4 55