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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: J Comp Neurol. 2020 Mar 26;528(14):2420–2444. doi: 10.1002/cne.24901

Figure 6. All measured parameters of GI motility are abnormal in 11-month-old Cdnf−/− mice.

Figure 6.

GITT, gastric emptying, and colonic expulsion time, were evaluated.All values shown are means determined from measurements made with Cdnf+/+ (n =6) and Cdnf−/− (n =6) mice at each age. (a) GITT increases as mice age in both Cdnf+/+ (green) and Cdnf−/− (magenta) mice; however, the degree to which transit slows as mice mature was significantly greater in Cdnf−/− than in Cdnf+/+ animals (2 way Anova F (1, 20) =34.9). (b) GITT (expressed as % of Cdnf+/+ GITT) in 3-month-old Cdnf+/+ mice was not significantly different from that of their Cdnf−/− littermates (two tailed t test t = 0.4463 df = 10 p = 0.6649); however, GITT in 11-month-old Cdnf−/− mice was significantly longer than in their Cdnf+/+ littermates (two-tailed t test t = 3.682 df = 8.491). (c) The time to expel a bead inserted into the rectum (colonic expulsion time, CET) in 3-month-old Cdnf+/+ mice (expressed as CET % Cdnf+/+) was not significantly different from that of their Cdnf−/− littermates (two-tailed t test t = 0.4594; df = 7.144 p = 0.6596); however, CET in 11-month-old Cdnf−/− mice was significantly greater than in their Cdnf+/+ littermates (two-tailed t test t = 2.866 df = 6.748). (d) The efficiency of gastric emptying (expressed as % Cdnf+/+ GE) in 3-month-old Cdnf+/+ mice was not significantly different from that in their Cdnf−/− littermates (two tailed t test t = 0.075587 df = 7.998 p = 0.9414); however, the efficiency of gastric emptyping in 11-month-old Cdnf−/− mice was substantially less than that in their Cdnf+/+ littermates (two-tailed t test t = 3.537; df = 3.221), that is, gastric emptying was delayed in the aged Cdnf−/− mice.