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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: J Comp Neurol. 2020 Mar 26;528(14):2420–2444. doi: 10.1002/cne.24901

Figure 8. CDNF promotion of dopaminergic neuronal development/survival in vitro is concentration-dependent and altered by the presence of non-neuronal cells.

Figure 8.

a. TH immunoreactivity was used as a dopaminergic neuronal marker and plotted as a function of the log of the concentration of CDNF. Non-linear regression analysis revealed that the data for isolated ENCDC. were best fit by a sigmoidal concentration-effect curve (3-parameter; Hill coefficient ~1). b. CDNF did not enhance the development of total neurons at any concentration tested [One way Anova F(4, 23) = 1.515] in immunoselected ENCDC. c. Although CDNF enhanced the development/survival of dopaminergic neurons in mixed cultures, the maximal effect was reached at 1 ng/ml, the lowest concentration tested, and a sigmoidal concentration-effect curve could not be obtained. d-e. The immunoreactivity of neurons (HuC/D; magenta) and TH (green) developing in the presence of vehicle (d) and CDNF (e) is illustrated. Coincidence of HuC/D and TH immunoreactivities marks the presence of dopaminergic neuron (arrows). f-i. Cultures of immunoselected ENCDC. The immunoreactivities of the neuronal marker, HuC/D (f), TH (g) and DAT (h) are coincident (l). j-k. Similar numbers of total neurons (HuC/D; magenta) develop from isolated ENCDC in the presence of vehicle (j) or CDNF (k). All bars = 25 μm.