Table 1:
PCR Primers, Conditions Used for Amplification, Expected Sizes of Products
| Primers/Gen Bank Accession Number | Sense (5’−3’) Nucleotides | Antisense (5’−3’) Nucleotides | Cycles (n) | Annealing Temp (°C) | Product (bp) |
|---|---|---|---|---|---|
| p75NTR/NM_033217 | CCGATACGGTGACCACTGTGATG | AGCAGCCAAGATGGAGCAATAGAC | 35 | 58 | 98 |
| 835–857 | 932–909 | ||||
| Hand1/NM_008213 | TTGAAGGCTCGACTCAAGGT | AGCGACAAGAAGGAAAACCA | 39 | 63 | 269 |
| 1266–1283 | 1534–1513 | ||||
| CDNF/NM_177647 | ATGCGGTGCATCAGTCCAACTGC | GAGCTCCGTTTGGGGGTATATC | 35 | 60 | 561 |
| 63–85 | 623–602 | ||||
| Foxa2/HNF-3β/ | GTATGCTGGGAGCCGTGAA | GGAGGTTTGGCGTGTGTGTAG | 39 | 63 | 463 |
| NM_010446 | 110–128 | 573–593 | |||
| β-actin/ NM_007393 | TGTTTGAGACCTTCAACACC | CAGTAATCTCCTTCTGCATCC | 30 | 57 | 588 |
| 448–467 | 1035–1015 | ||||
| DAT/NM_010020 | CTTCTCCTCTGGCTTCGTTGT | CAGGGTAGATGATGAAGATCAACC | qPCR | qPCR | 116 |
| 1225–1245 | 1340–1317 | ||||
| GAPDH/NM_008084.2 | TGATGGGTGTGAACCACGAG | GGCATGGACTGTGGTCATGAG | qPCR | qPCR | 146 |
| 439–458 | 584–564 |