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Functionality of individual dCas9 proteins using the Cas beacon assay. (A) Binding of a dCas9 fusion protein to a Cas beacon target and the subsequent displacement of the quencher strand. (B) Binding efficiencies of three different dCas9 fusion proteins to their corresponding Cas beacon targets. In all cases, dCas9 binding resulted in a significant increase in fluorescence. Blue: SPdCa s9, Green: ST1dCas9, and Red: SadCas9.
