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. Author manuscript; available in PMC: 2020 Dec 9.
Published in final edited form as: Chem Commun (Camb). 2019 Jul 11;55(57):8219–8222. doi: 10.1039/c9cc04002a

Figure 3.

Figure 3.

(A) Schematic of the dual beacon assay. (A) Fully hybridized dual beacon has only background fluorescence. Addition of dCas9A/sgRNA complex results in displacement of the quencher Oligo 2 and an increase in FAM fluorescence. While incubation with dCas9B:/sgRNA complex displaces only Oligo 4 resulting in Cy5 fluorescence. When both dCas9 fusion proteins and the cognate sgRNAs are present, both quencher strands are displaced causing an increase in both FAM and Cy5 fluorescence. (B) Binding efficiencies of three dCas9 fusion protein combinations to their corresponding dual beacon targets. (B) SpdCas9/StidCas9, (C) SpdCas9/SadCa9Green, (D) SadCas9/St1dCas9.