Skip to main content
. Author manuscript; available in PMC: 2020 Dec 9.
Published in final edited form as: Chem Commun (Camb). 2019 Jul 11;55(57):8219–8222. doi: 10.1039/c9cc04002a

Figure 5.

Figure 5.

Conditional assembly of a two-component cellulosome. (A) Binding of SpdCas9-CBD is activated by a RNA trigger to unblock the spacer region of thgRNA by toehold-mediated strand displacement. (B) Conditional binding of SpdCas9-CBD using the Cas beacon assay. Binding was performed using either sgRNA or thgRNA. Activation was observed with the thgRNA only when a corresponding RNA trigger was added. (C) Cellulose hydrolysis by conditional cellulosome formation by toehold-mediated strand displacement. Cellulosomes assembled using sgRNA and the activated thgRNA produced similar reducing sugar titers while the inactivated thgRNA produced less reducing sugars comparable to the unassembled enzymes in Fig. 5.